The accelerated evolution of the AI era is profoundly reshaping the underlying logic of organizational survival and development, and the traditional industrial paradigm dominated by competition is facing diminishing returns in value creation efficiency. Based on the biological principle of “interaction effects”, this paper reconstructs the theoretical connotation of the symbiotic concept and proposes a new path for organizational paradigm reconstruction driven by the symbiotic concept. By systematically deconstructing the theoretical framework of symbiotic organizations, this paper reveals their evolutionary path of “mutual subjectivity–interaction effects–value co-creation–holistic evolution”, and elucidates the dynamic mechanism through which organizations achieve nonlinear value amplification via internal and external synergistic interdependencies. The study identifies three core characteristics of symbiotic organizations: transcending the one-dimensional goal of profit maximization with meaning maximization, achieving internal-external synergy through dynamic boundary maintenance, and building enhanced system resilience through symbiotic intensity. This paper provides a theoretical paradigm and analytical tools for organizational paradigm innovation in the AI era, offering significant insights for overcoming the challenges of enterprise transformation.